Students' RESEARCH project on:

Photo-Dynamic Therapy (PDT)

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Research Interns: Jitesh Neupane, Lilamber Chaudhary, Safal Sinnya, Sajina Shakya,        

                               Sabitri Ghimire; Bachelor in Biomedical Engineering, CBEAS*

Supervisors:   Mr. Vishwa Prakash Shrivastava, Principal, CBEAS

Mr. Niranjan Prasad Shah, Asst. Prof, Medical Biotechnologist, Institute of Medicine (IOM), Kathmandu, Nepal, and

Mr. Dinesh Madhup, Electrical and Electronics Engineer, Dhulikhel, Kavre, Nepal

Abstract:

Photo Dynamic therapy (PDT) has been widely used to treat various diseases including autoimmune diseases. LASER is mostly in use as the source of light during PDT, but having been aware of laser’s negative effects we propose to use  Light Emitting Diodes (LEDs) of variable intensity and wavelength (may be red, white, or Infra red to name a few) as the source of light. The aim of this study is to investigate the cellular response of T-cells after PDT. LEDs of different wavelengths will be chosen to investigate the most appropriate intensity and wavelength that can penetrate the tissue without producing heating or burning effect. Duration and frequency will also be taken into consideration in order to investigate the cellular responses. A constant current and voltage source will be supplied to maintain the required intensity (The intensity of light emitted by these LEDs is directly proportional to the magnitude of the current supplied). In doing so, the temperature should not exceed the physiological requirement. The so produced light is desired to be supplied to suppress or kill, especially, T-cells, which are responsible for autoimmune diseases and inflammatory responses. Furthermore, this technique may be used to investigate the effects in treating skin diseases, to detoxify an organism, to stimulate the healing process in an organism which is ill, diseased or injured; aiding in the elimination of pain, and to treat burn or scar tissues. The photo receptors in our cells moderate the light input, so, there is a self limiting effect, preventing abuse when the correct light source parameters are first selected. Defective or cancer cells are said to lack that protective enzyme and thus can be killed by the